ERA-HDHL cofunded call “Biomarkers for Nutrition and Health” (BioNH 2016)

“Innovative Technological Approaches for validation of Salivary AGEs as novel biomarkers in evaluation of risk factors in diet-related diseases”
SALIVAGES
2017-05-01
2020-04-30
MASSIMO COLLINO
Partner Organization Partner Country

1. Overall project description


1.1 Summary

Recent findings convincingly demonstrate that Advanced Glycation End Products (AGEs) are modifiable by diet and reflect changes in healthy state. SALIVAGES investigates whether that diet-induced AGEs can act as reliable biomarkers of changes in health status and/or risk, focusing on the most highly accessible source of AGEs, the saliva. SALIVAGES will provide innovative and original tools for assessing status and efficacy of interventions, namely biosensors, metabolomics and biomimic approaches.


1.2 Highlights

We have identified potential correlations between salivary biomarkers/fineprints and diet-driven changes in metabolic status. The results so far obtained in terms of developments of new methods for synthesis and characterization of advanced glycation end products (AGEs) and related salivary volatilome and advanced glyco-signaturing, are paving the way for the validation of salivary AGES as reliable early and non-invasive markers of health status. We are now developing prototypes of biosensors with two components: electrochemical and optical componenents for AGEs detection.


4. Impact


4.1 List of publications

AuthorsTitleYear, Issue, PPDoiPdf
Aranka Ilea, Anida M. Băbţan, Bianca A. Boşca, Maria Crişan, Nausica B. Petrescu, Massimo Collino, Rosa M. Sainz, Jared Q. Gerlach, Radu Septimiu Câmpian.(partners involved: University of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO University of Turin, IT University of Oviedo, SP National University of Ireland Galway, IR)Advanced glycation end products (AGEs) in oral pathology.2018, 93, 22 https://doi.org/10.1016/j.archoralbio.2018.05.013
Băbţan AM, Ilea A, Boşca BA, Crişan M, Petrescu NB, Collino M, Sainz RM, Gerlach JQ, Câmpian RS. (prtners involved: University of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO University of Turin, IT University of Oviedo, SP National University of Ireland Galway, IR)Advanced glycation end products as biomarkers in systemic diseases: premises and perspectives of salivary advanced glycation end products.2019 doi: 10.2217/bmm-2018-0448.
Collotta D, Lucarini L, Chiazza F, Cento AS, Durante M, Sgambellone S, Chini J, Baratta F, Aragno M, Mastrocola R, Masini E, Collino M. (Partner involved: University of Turin, IT )Reduced Susceptibility to Sugar-Induced Metabolic Derangements and Impairments of Myocardial Redox Signaling in Mice Chronically Fed with D-Tagatose when Compared to Fructose. 2018 doi: 10.1155/2018/5042428.
Feeney, S., Gerlach, J. Q., Slattery, H., Kilcoyne, M., Hickey, R. M., & Joshi, L. (Partner involved National University of Ireland Galway, IE)Lectin microarray profiling and monosaccharide analysis of bovine milk immunoglobulin G oligosaccharides during the first 10 days of lactation2019 https://doi.org/10.1002/fsn3.950
Tertis M., Melinte G., Ciui B.C., Simon I., Stiufiuc R., Sandulescu R., Cristea C. (Partner involved University of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO) A Novel Label Free Electrochemical Magnetoimmunosensor for Human Interleukin-6 Quantification in Serum, 2019, 31, 28210.1002/elan.201800620.
Tiuca Gug I., Tertis M., Hosu O., Cristea C., (Partner involved University of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO) Salivary biomarkers detection: Analytical and immunological methods overview, 2019, 113, 301https://doi.org/10.1016/j.trac.2019.02.020.
Ciui B, Tertis M, Feurdean C, Ilea A, Sandulescu R, Wang J, Cristea C, (Partner involved University of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO) Cavitas electrochemical sensor toward detection of N-epsilon(Carboxymethyl)lysine in oral cavity, 2019, 281, 399https://doi.org/10.1016/j.snb.2018.10.096.
Ilea A, Andrei V, Feurdean CN, Băbțan AM, Petrescu NB, Câmpian RS, Boșca AB, Ciui B, Tertiș M, Săndulescu R, Cristea C. (Partner involved University of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO)Saliva, a Magic Biofluid Available for Multilevel Assessment and a Mirror of General Health-A Systematic Review. 2019, 9
Petrescu Bianca Nausica, Băbțan Anida-Maria, Olga Sorițău, Buhățel Dan, Ionel Anca, Feurdean Nicoleta Claudia, Boșca Adina Bianca, Câmpian Radu Septimiu, Ilea Aranka. (Partner involved University of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO) Stem Cells in Head and Neck Cancers Pathogenesis: Are Advanced Glycation End Products (AGEs) Involved? 2019, 4 DOI: 10.21767/2471-8084.100061

4.2 Presentation of the project

Target groupAuthorsMeans of communicationHyperlinkPdf
scientistsAdina Bianca Boșca, Marian Tăulescu, Viorel Miclăuș, Anida Maria Băbțan, Aranka Ilea. Accumulation of N-epsilon carboxymethyl lysine in rat oral cavity tissues: a biomarker of aging. 23rd BaSS Congress, Dental Medicine of the Younger vs. Elderly Patients, 10 – 12 May 2018, PALAS Congress Hall, IASI, ROMANIA, pp. 55Oral presentation
scientistsCento Alessia S.; Chiazza F; Collotta D; Barutta F; Aragno M; Scheijen J; Collino M, Gaens KH; Schalkwijk CG; Wouters K; Mastrocola R. Genetic deletion of RAGE in Dbdb mice interferes with AGEs receptors and detoxifying systems in liver. Conference: 19th Biennial Meeting of the Society-for-Free-Radical-Research-International (SFRRI) Location: Lisbon, PORTUGAL Date: 2018Oral presentation
scientistsChiara Cordero,1*Erica Liberto1, Fausto Chiazza1, Raffaella Mastrocola2, Stephen E. Reichenbach3, Carlo Bicchi1 and Massimo Collino SALIVAGE: The “form” makes the difference: unrevealing the differential impact of liquid and solid fructose in mice urinary metabolic signatures by comprehensive two-dimensional gas chromatography XII Italian Food Chemistry Congress (Camerino, September 24-27 2018)Oral presentation
scientistsJared Gerlach, Raffaella Mastrocola, Erica Liberto, Chiara Cordero, Massimo Collino and Lokesh Joshi SALIVAGES: Lectin microarray-based investigation of diet dependent, differential glycosylation of proteins in murine biological fluids and organ homogenates XII Italian Food Chemistry Congress (Camerino, September 24-27 2018)Oral presentation
scientistsOral presentation
scientistsHellwig M, Manig F, Pietz F, Henle T: Salivary methionine sulfoxide as a marker for oxidative stress. International Conference on Oxidative Stress Reduction, Redox Homeostasis and Antioxidants - Redox 2018, Paris, June 25-26 2018Oral presentation
scientistsManig F, Hellwig M, Pietz F, Henle T: Surveying the salivary AGEom - quantitation of glycation compounds in saliva and the dietary impact of an AGE-rich diet. 13. International Symposium on the Maillard Reaction, Montreal, September 10-14 2018 Chiara Cordero, Marta Cialiè Rosso, Erica Liberto, Massimo Collino, Gianluca Aimaretti, Paolo Marzullo, Chiara Mele, Stephen E. Reichenbach, Qingping Tao, Carlo Bicchi (2018). EXPLORING “EXTRA-DIMENSIONS” TO CAPTURE METABOLITE FINGERPRINTS IN METABOLICALLY HEALTHY AND UNHEALTHY OBESE PATIENTS BY GC×GC TANDEM IONIZATION-TOFMS. In: Abstract Book. p. 16, Messina Italia:Luigi Mondello and Paola Dugo, ISBN: 978-88-941816-1-6, Riva del Garda (TN) Italia, May 13 – 18, 2018Oral presentation
scientistsHellwig M, Manig F, Henle T: Yeast metabolites of glycated amino acids in beer – potential biomarkers in saliva and urine? XII Italian Food Chemistry Congress, Camerino, September 24-27 2018Oral presentation
scientistsAdina Bianca Bosca, Marian Tăulescu, Viorel Miclăuş, Mihai Negru, Anida Maria Babtan, Nausica Bianca Petrescu, Anca Mesaros, Cristina Gasparik, Alexandra Irimie, Alina Elena Pârvu, Maria Crişan, Aranka Ilea. ACCUMULATION OF N-EPSILON CARBOXYMETHYL LYSINE IN RAT ORAL CAVITY TISSUES AND ORGANS CORRELATED WITH AGING. International Symposium: Health through education, prevention and treatment, 7-8 December, 2018, Cluj-Napoca, Romania Anida-Maria Babtan, Aranka Ilea, Nausica Petrescu, Bianca Bosca, Maria Crișan, Manuela Lenghel, Anca Ionel, Andreea Pop, Willi Uriciuc, Codruta Mirica, Claudia Feurdean, Radu Septimiu Campian. ADVANCED GLYCATION END PRODUCTS (AGEs) ULTRASOUND EVALUATION IN FACIAL SKIN AND MUCOSA. International Symposium: Health through education, prevention and treatment, 7-8 December, 2018, Cluj-Napoca, RomaniaOral presentation
scientistsBoșca Adina Bianca, Șovrea Alina Simona, Miclăuș Viorel, Ruxanda Flavia, Mihu Carmen Mihaela, Melincovici Carmen, Constantin Anne Marie, Buhățel Dan, Băbțan Anida-Maria, Petrescu Bianca Nausica, Câmpian Radu Septimiu, Pârvu Alina Elena, Ilea Aranka. New data concerning the origin and activity of the multinucleated giant cells in oral cavity granulomas. The XVIth National Symposium on Microscopic Morphology with International Participation, Craiova 23-26 May 2018, Abstract Summaries pp. 26-27Oral presentation
scientistsWilli A. Uriciuc, Aranka Ilea, Horatiu Vermeșan, Radu Septimiu Câmpian. Research about different methods in dental alloys casting structures. Napoca Biodent Symposium 8th Edition, Dentistry – from research to practice, 25-26 May, 2018, Cluj-Napoca, RomaniaOral presentation
scientistsCollino M, Liberto E, Mastrocola R, Ferrocino I, Chiazza F, Cento AS, Collotta D, Rantsiou K, Ilea A, Aragno M, Cocolin L, Bicchi C, Cordero C. The “form” makes the difference: unrevealing the differential susceptibility to sugar-induced metabolic derangements evoked by liquid and solid fructose formulations. EuroNutrition 2018. Annual Congress on Nutrition & Healthcare, October 18-20, 2018 Paris, FranceInvited Talk
scientistsM. Collino “Innovative Technological Approaches for validation of Salivary AGEs as novel biomarkers in evaluation of risk factors in diet-related diseases” EuroNutrition 2018. Annual Congress on Nutrition & Healthcare, October 18-20, 2018 Paris, FranceInvited Talk
scientistsCollino M, Liberto E, Mastrocola R, Ferrocino I, Chiazza F, Cento AS, Collotta D, Rantsiou K, Ilea A, Aragno M, Cocolin L, Bicchi C, Cordero SALIVAGE: The “form” makes the difference: unrevealing the differential susceptibility to sugar-induced metabolic derangements evoked by liquid and solid fructose formulations. XII Italian Food Chemistry Congress (Camerino, September 24-27 2018)Poster presentation
scientistsLiberto E*, Cordero C, Mastrocola R, Ferrocino I, Chiazza F, Cento AS, Collotta D, Rantsiou K, Aragno M1, Cocolin L, Bicchi C and Collino M SALIVAGE: The “form” makes the difference: comprehensive two‐dimensional gas chromatography as highly informative fingerprinting tool to study the impact of fructose formulation on mice’s microbiota and its volatile metabolome signatures XII Italian Food Chemistry Congress (Camerino, September 24-27 2018)Poster presentation
scientistsManig F, Pietz F, Hellwig M, Henle T: Surveying the salivary AGEome - quantitation of glycation compounds in saliva and the impact of an AGE-rich diet. XII Italian Food Chemistry Congress, Camerino, September 24-27 2018Poster presentation
scientistsManig F, Pietz F, Hellwig M, Henle T: AGEs in saliva: indication for the metabolic fate of Maillard reaction products in vivo. 47. German Food Chemistry Congress, Berlin, September 17-19 2018Poster presentation
scientistsAdina Bianca Boșca, Marian Tăulescu, Viorel Miclăuș, Mihai Negru, Anida Maria Băbțan, Nausica Bianca Petrescu, Dan Buhățel, Anca Mesaros, Cristina Gasparik, Alexandra Irimie, Alina Elena Parvu, Maria Crișan, Massimo Collino, Aranka Ilea. Expression of N-epsilon carboxymethyl lysine in rat oral cavity tissues and organs: a biomarker of aging. CHIMALI, XII Italian Food Chemistry Congress, Camerino, Italy, September 24-27, 2018Poster presentation
scientistsIlAnida-Maria Băbţan, Aranka Ilea, Nausica Petrescu, Bianca Boşca, Maria Crişan, Manuela Lenghel, Anca Ionel, Dan Buhăţel, Arin Sava, Andreea Pop, Willi Uriciuc, Codruţa Mirica, Claudia Feurdean, Radu Septimiu Câmpian. EVALUATION OF AGEs AT THE SKIN AND MUCOSA LEVELS IN THE OROMAXILOFACIAL AREA THROUGH ULTRASOUNDS. Days of "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, 7 December, 2018, Cluj-Napoca, Romania, p. 88ea Aranka, Crișan Maria, Boșca Adina Bianca, Constantin Anne-Marie, Băbțan Anida Maria, Petrescu Nausica Bianca, Buhățel Dan, Ionel Anca, Câmpian Radu Septimiu. High frequency ultrasound assessment in cutaneous senescence process. CHIMALI, XII Italian Food Chemistry Congress, Camerino, Italy, September 24-27, 2018Poster presentation
scientistsClaudia Feurdean, Aranka Ilea, Ondine Lucaciu, Anca Ionel, Dan Buhajel, Arin Sava, Nausica Petrescu, Adina Sirbu, Andreea Pop, Roxana Bordea, Willi Uriciuc, Codruta Mirica, Anida-Maria Băbţan, Radu Septimiu Câmpian. INSERTION OF SALIVARY BIOSESORS IN DENTAL PROSTHESES. Days of "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, 7 December, 2018, Cluj-Napoca, Romania, p. 90Poster presentation
scientistsBoșca Adina Bianca, Dinte Elena, Ilea Aranka, Muntean Dana, Tomuta Ioan, Hangan Dana, Pârvu Alina Elena, Mihu Carmen Mihaela, Melincovici Carmen, Șovrea Alina, Constantin Anne Marie, Băbțan Anida, Petrescu Nausica, Câmpian Radu Septimiu. Clinical efficacy of a mucoadhesive nanoparticulate gel based on antibiotics, anti-inflammatory drugs and anthocyanins used in the periodontal treatment. The 22nd International Exhibition of INVENTICS – ”INVENTICA 2018” Iași, 27-29 June 2018, Iași, RomaniaPoster presentation
scientistsV Cepas, F Manig, R Matesanz, M Hellwig, T Henle, RM Sainz: Some dietary Advanced Glycation Endproducts (AGEs) reduce cell viability and produce Reactive Oxygen Species. XII Italian Food Chemistry Congress (CHIMALI 2018). Camerino (Italy) Sept 2018Poster presentation
scientistsV Cepas, R Matesanz, F Manig, M Hellwig, T Henle, RM Sainz: Dietary Glycation Endproducts (AGEs) are cytotoxic for keratinocytes. 41 Congreso de la Sociedad Española de Bioquímica y Biología Molecular. Santander (Spain) Sept 2018Poster presentation
Students (Professional training school)Vanesa Cepas. Sugars and metabolic disorders. Internatinal Day of Women and Girls in Science. Professional training school CIFP Avilés (Spain) February 2019Oral presentation
Students (Elementary School)Vanesa Cepas. From science to the table. What molecules are we eating in our foods? International Day of Women and Girls in Science. Elementary School San Pedro de Los Arcos. Oviedo (Spain) February 2019Oral presentation
Students (Secondary School)Vanesa Cepas. Sugar and metabolic disorders. Secondary School Virgen de La Luz. Avilés (Spain) November 2018Oral presentation

4.3 List of submitted patents and other outputs

Patent licencePartners involvedYearInternational eu or national patentCommentPdf
Salivary optical sensor made by lateral coupling of an optical emission fiber on the surface and a fluorescent optical fiber integrated into an intraoral deviceUniversity of Medicine and Pharmacy ”Iuliu Hațieganu” Cluj-Napoca, RO2019National Patent Patent application No - OSIM A00136/27.02.2019

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