Carb-Q-4-Health
Tailored Carbohydrate Quality for Personalized Weight Management and Metabolic Health
This project investigates the role of carbohydrate quality (CQ) on weight management, appetite and cardiometabolic health in humans as well as underlying mechanisms by which CQ affects consumers. It will also promote career development and mobility of Early Career Scientists (ECS) to foster the new generation of scientists in food and health. Experimental models, clinical approaches and OMICs are used in a multidisciplinary setting. Results will be transferred to industry and disseminated to the scientific community and general public. We take advantage of the data and samples from 12-wk weight-loss study conducted in collaboration with Agrifood industry, investigating weight- and fat-loss after a high fibre vs refined fibre diet. Satiety and gut microbiota have been evaluated as determinants of weight-loss and are analyzing cardiometabolic risk factors and molecular phenotypes associated with weight-loss and cardiometabolic risk profiles using metabolomics and proteomics. Molecular signatures of successful weight-loss and low cardiometabolic risk, will be validated in a prospective cohort of women (n=5022, data already available), and in a corresponding cohort in men (n=5000). Moreover, a rat model has been set up and used to study the potential adverse effects of refined carbohydrates at organ/tissue level and the potential beneficial effects of supplementation with fermentable dietary fibre. Preliminary data suggest adverse effects at a molecular level induced by fructose and galactose, which are to some extent mitigated by addition of fermentable fibre in the diets. Particular focus has been put on studies of metabolic inflammation and effects mediated through AGEs, the gut barrier and microbiota. We will compare preclinical metabolite profiles to metabolic signatures of the human studies to elucidate the molecular alterations associated with weight-loss and cardiometabolic risk in humans (not yet started).
In summary, have/are exploiting (i) metabolomics to discover biomarker panels that can predict tailored advice on high-fibre diets for successful weight-loss (personalized nutrition), (ii) mechanistic studies to identify molecular mechanisms evoked by carbohydrates and (iii) the relationship between perceived satiety and food structure. Results are expected to translate into improved high-fibre foods tailored for optimal metabolic health and weight management for individuals or consumer groups.
Consortium
| Partner Organization | Partner Country |
|---|---|
| Università degli Studi di Torino | Italy |
| University of Barcelona | Spain |
| University of Hohenheim | Germany |
| Bellvitge Biomedical Research Institute (IDIBELL) | Spain |
| SLU-Swedish University of Agricultural Sciences | Sweden |
Highlights
2021
- The microstructure of in vitro digested wheat and rye products was very different, with a higher degree of disintegration on wheat products. This will be of relevance when combining with results of metabolomics analysis from the intervention studies.
2022
- Rye, compared to wheat, induced some changes in gut microbiota composition, including increased abundance of the butyrate producing Agathobacter and reduced abundance of [Ruminococcus] torques group, which may be related to reductions in low grade inflammation caused by the intervention. Plasma butyrate increased in the rye group. Our data show that an intervention with high fiber rye foods induced some changes in gut microbiota composition and plasma short chain fatty acid concentration, which were associated with improvements in metabolic risk markers as a result of the intervention.
- We found that self-reported appetite measurements under free-living conditions show similar results as self-reported measures under tightly controlled clinical conditions. This suggest that self-reported appetite measures conducted in large scale studies in home-setting works well and could be applied in personalized nutrition studies.
- A diet high in fructose resulted in adverse metabolic effects compared with isocaloric control at organ level. The effect was parallelled by adverse effects on inflammation markers, blood lipid profiles and AGEs.
- We found a difference in metabolites after high fibre rye intervention vs refined wheat intervention, but none of the metabolites predicted weight-loss.
- The improvements in the targeted-metabolomic methodology would allow a more comprehensive assessment of the gut microbiota metabolism of dietary components, and a more accurate and robust quantification
- Human weight-loss trial plasma samples have been processed and analyzed by UHPLC-MS/MS
- 14 monthly Journal Club meeting sessions and 6 bimonthly ECS webinars have been held
Products
Author: MhD Omar NA*, Dicksved J, Kruger J, Zamaratskaia G, Michaelssson K*, Wolk A*, Frank J*, Landberg R*:
Link: https://doi.org/10.3389/fnut.2022.922336
Author: Raffaella Mastrocola, Debora Collotta*, Giulia Gaudioso, Marie Le Berre, Alessia Sofia Cento, Gustavo Ferreira Alves* , Fausto Chiazza* , Roberta Verta , Ilaria Bertocchi, Friederike Manig, Michael Hellwig, Francesca Fava , Carlo Cifani , Manuela Aragno, Thomas Henle, Lokesh Joshi, Kieran Tuohy and Massimo Collino*
Link: https://doi.org/10.3390/nu12092497
Author: Kia Nøhr Iversen*, Johan Dicksved, Camille Zoki, Rikard Fristedt, Erik A Pelve, Maud Langton*, Rikard Landberg*
Link: https://doi.org/10.3390/nu14081669
Author: Nor Adila Mhd Omar*, Jan Frank*, Johanita Kruger*, Federica Dal Bello*, Claudio Medana*, Massimo Collino*, Galia Zamaratskaia*, Karl Michaelsson*, Alicja Wolk*, Rikard Landberg*
Link: https://doi.org/10.1002/mnfr.202001133
Author: Fabian Lanuza 1, Raul Zamora-Ros *, Nicole Hidalgo-Liberona , Cristina Andrés-Lacueva *, Tomás Meroño*;
Link: https://doi.org/10.3390/nu12092815
Author: Tor-Roca A, Garcia-Aloy M, Mattivi F, Llorach R, Andres-Lacueva C*, Urpi-Sarda M.
Link: https://doi.org/10.1021/acs.jafc.0c04387
Author: Fidèle Almasri, Nadine Sus, Debora Collotta, Raffaella Mastrocola, Massimo Collino, Jan Frank,
Link: https://doi.org/10.1093/cdn/nzac068.002
Communication & Dissemination Activities
| Target group | Authors | Means of communication | Hyperlink |
|---|---|---|---|
| Scientists, industry and health care staff | Landberg, Rikard. Metabolomics to guide personalized cereal food consumption - emerging results from human studies. HealthGrainForum Webinar on "Personalisation of Grain Consumption" September 16, 2020. | Invited webinar presentation | |
| Scientists, industry | Landberg, Rikard. The role of grains in sustainable & personalized nutrition 4 health. HealthGrainForum autumn meeting, November 5-6 | Invited webinar presentation | |
| Scientists | Anne K Eriksen , Carl Brunius , Mohsen Mazidi , Per M Hellström , Ulf Risérus , Kia N Iversen, Rikard Fristedt , Li Sun , Yi Huang, Natalja P Nørskov, Knud Erik B Knudsen , Cecilie Kyrø , Anja Olsen , Anne Tjønneland, Johan Dicksved, Rikard Landberg. Effects of whole-grain wheat, rye and lignan supplementation on cardio-metabolic risk factors in men with metabolic syndrome: a randomized crossover trial. Nordic Nutrition Conference, Helsinki 15-16 December 2020. | Invited oral presentation at the conference (through zoom) | |
| Industry and scientist | Industry and scientists Landberg, Rikard. Rye, gut microbiota and cardio-metabolic health. Symposium: Rye-The “wholy” grain for gut and brain, Nordic Nutrition Conference 15 December 2020 | Invited oral presentation at the symposium | |
| Industry | Cristina Andres-Lacueva, polyphenol and biomarkers assement, July 15th, 2020 | Invited oral, webinar, industrial training, online | |
| Scientist , Open access | Pol Castellano Ontology, FOBI, POMA | Invited webinar | |
| Scientist, Industry, health care staff | Tomas Meroño, Andrea Unión Caballero, Cristina Andrés-Lacueva. Importance of omics sciences in biotechnology. III Congress of Food science, Nutrition and Dietetics organized by the Spanish Academy of Nutrition and Dietetics. 23-27 November 2020. | Invited webinar presentation at the symposium | |
| Scientists | American Society for Nutrition- NUTRITION 2022 “Preliminary findings on the effects of high intakes of fructose and galactose with or without fructooligosaccharides on inflammatory markers and blood lipids in rats” Fidèle Almasri, Nadine Sus, Debora Collotta, Raffaella Mastrocola, Massimo Collino, Jan Frank | Online Poster | |
| Scientists | Fidèle Almasri, Nadine Sus, Debora Collotta, Raffaella Mastrocola, Massimo Collino, Jan Frank. Max Rubner-Institut “Preliminary findings on the effects of high intakes of fructose and galactose with or without fructooligosaccharides on inflammatory markers in rats” | Online poster | |
| Scientists | Collotta D., Almasri F., Murzio C., Aimaretti E., Aragno M., Mastrocola R., Frank J. Collino M. 15th World Congress on Inflammation.5-8 June 2022. Sugar-induced metaflammation: a comparative analysis between dietary fructose and galactose and protective effects evoked by prebiotic fructooligosaccharides in rats. | Oral Presentation | |
| Scientists | Collotta Debora, Mastrocola Raffaella, El-Masri Fidele, Ferreira Alves Gustavo, Aimaretti Eleonora, Aragno Manuela, Porchietto Elisa, Frank Jan, Collino Massimo 42 Congresso Nazionale della Società Italiana di Farmacologia- 16-19th November 2022 Rome Protective effects of prebiotic fructoligosaccharides supplementation in counteractiong metabolic derangements evoked by chronic exposure to sugars (fructose versus galactose). | Oral presentation | |
| Scientists | -3rd Annual Congress on Food Science Nutrition and Women’s Health, December 6th,2022 Protective effects of prebiotic fructooligosaccharides supplementation in counteracting metabolic derangements evoked by chronic exposure to sugars (fructose versus galactose). Collotta Debora, Mastrocola Raffaella, El-Masri Fidele, Ferreira Alves Gustavo, Aimaretti Eleonora, Aragno Manuela, Porchietto Elisa, Frank Jan, Collino Massimo | Oral online presentation |