Tailored Carbohydrate Quality for Personalized Weight Management and Metabolic Health
This project investigates the role of carbohydrate quality (CQ) on weight management, appetite and cardiometabolic health in humans as well as underlying mechanisms by which CQ affects consumers. It will also promote career development and mobility of Early Career Scientists (ECS) to foster the new generation of scientists in food and health. Experimental models, clinical approaches and OMICs are used in a multidisciplinary setting. Results will be transferred to industry and disseminated to the scientific community and general public. We take advantage of the data and samples from 12-wk weight-loss study conducted in collaboration with Agrifood industry, investigating weight- and fat-loss after a high fibre vs refined fibre diet. Satiety and gut microbiota have been evaluated as determinants of weight-loss and are analyzing cardiometabolic risk factors and molecular phenotypes associated with weight-loss and cardiometabolic risk profiles using metabolomics and proteomics. Molecular signatures of successful weight-loss and low cardiometabolic risk, will be validated in a prospective cohort of women (n=5022, data already available), and in a corresponding cohort in men (n=5000). Moreover, a rat model has been set up and used to study the potential adverse effects of refined carbohydrates at organ/tissue level and the potential beneficial effects of supplementation with fermentable dietary fibre. Preliminary data suggest adverse effects at a molecular level induced by fructose and galactose, which are to some extent mitigated by addition of fermentable fibre in the diets. Particular focus has been put on studies of metabolic inflammation and effects mediated through AGEs, the gut barrier and microbiota. We will compare preclinical metabolite profiles to metabolic signatures of the human studies to elucidate the molecular alterations associated with weight-loss and cardiometabolic risk in humans (not yet started).
In summary, have/are exploiting (i) metabolomics to discover biomarker panels that can predict tailored advice on high-fibre diets for successful weight-loss (personalized nutrition), (ii) mechanistic studies to identify molecular mechanisms evoked by carbohydrates and (iii) the relationship between perceived satiety and food structure. Results are expected to translate into improved high-fibre foods tailored for optimal metabolic health and weight management for individuals or consumer groups.
|Università degli Studi di Torino
|University of Barcelona
|University of Hohenheim
|Bellvitge Biomedical Research Institute (IDIBELL)
|SLU-Swedish University of Agricultural Sciences
- The microstructure of in vitro digested wheat and rye products was very different, with a higher degree of disintegration on wheat products. This will be of relevance when combining with results of metabolomics analysis from the intervention studies.
- Rye, compared to wheat, induced some changes in gut microbiota composition, including increased abundance of the butyrate producing Agathobacter and reduced abundance of [Ruminococcus] torques group, which may be related to reductions in low grade inflammation caused by the intervention. Plasma butyrate increased in the rye group. Our data show that an intervention with high fiber rye foods induced some changes in gut microbiota composition and plasma short chain fatty acid concentration, which were associated with improvements in metabolic risk markers as a result of the intervention.
- We found that self-reported appetite measurements under free-living conditions show similar results as self-reported measures under tightly controlled clinical conditions. This suggest that self-reported appetite measures conducted in large scale studies in home-setting works well and could be applied in personalized nutrition studies.
- A diet high in fructose resulted in adverse metabolic effects compared with isocaloric control at organ level. The effect was parallelled by adverse effects on inflammation markers, blood lipid profiles and AGEs.
- We found a difference in metabolites after high fibre rye intervention vs refined wheat intervention, but none of the metabolites predicted weight-loss.
- The improvements in the targeted-metabolomic methodology would allow a more comprehensive assessment of the gut microbiota metabolism of dietary components, and a more accurate and robust quantification
- Human weight-loss trial plasma samples have been processed and analyzed by UHPLC-MS/MS
- 14 monthly Journal Club meeting sessions and 6 bimonthly ECS webinars have been held
Author: MhD Omar NA*, Dicksved J, Kruger J, Zamaratskaia G, Michaelssson K*, Wolk A*, Frank J*, Landberg R*:
Author: Raffaella Mastrocola, Debora Collotta*, Giulia Gaudioso, Marie Le Berre, Alessia Sofia Cento, Gustavo Ferreira Alves* , Fausto Chiazza* , Roberta Verta , Ilaria Bertocchi, Friederike Manig, Michael Hellwig, Francesca Fava , Carlo Cifani , Manuela Aragno, Thomas Henle, Lokesh Joshi, Kieran Tuohy and Massimo Collino*
Author: Kia Nøhr Iversen*, Johan Dicksved, Camille Zoki, Rikard Fristedt, Erik A Pelve, Maud Langton*, Rikard Landberg*
Author: Nor Adila Mhd Omar*, Jan Frank*, Johanita Kruger*, Federica Dal Bello*, Claudio Medana*, Massimo Collino*, Galia Zamaratskaia*, Karl Michaelsson*, Alicja Wolk*, Rikard Landberg*
Author: Fabian Lanuza 1, Raul Zamora-Ros *, Nicole Hidalgo-Liberona , Cristina Andrés-Lacueva *, Tomás Meroño*;
Author: Tor-Roca A, Garcia-Aloy M, Mattivi F, Llorach R, Andres-Lacueva C*, Urpi-Sarda M.
Author: Fidèle Almasri, Nadine Sus, Debora Collotta, Raffaella Mastrocola, Massimo Collino, Jan Frank,