MaPLE

Gut and blood microbiomics for studying the effect of a polyphenol-rich dietary pattern on intestinal permeability in the elderly

An increased intestinal permeability (IP), also known as leaky gut, could be responsible for activation of the immune system and inflammation, a common mechanism in many chronic age-related diseases.

The MaPLE project tested the hypothesis that an increased intake of polyphenol-rich foods can reduce IP and the quantity of inflammogenic bacterial factors in the bloodstream, promoting a protective metabolic phenotype. A dietary protocol was developed to deliver three portions per day of specific polyphenol-rich foods/beverages (berries and derived products, blood oranges and juice, pomegranate juice, Renetta apple and purée, green tea and dark chocolate products) to older participants living in a controlled setting (i.e. nursing home and residencies).

The polyphenol-enriched diet caused reductions in IP (reduced serum zonulin) and the effect was greatest in participants with higher baseline IP and metabolic dysregulation. Moreover, a reduction of diastolic blood pressure was found.Food metabolome profiles were characterized by an increase of flavanols (present in cocoa), the flavanones hesperetin and naringenin (present in oranges) and specific products of microbial metabolism, such as coumaric acids and urolithins (derived from blueberries and pomegranate). After polyphenol-rich diet intervention, the reductions in IP (serum zonulin) were associated with increases in theobromine and two methylxanthine metabolites. We were unable to detect significant changes in the overall faecal metabolites. However, it is noteworthy that, following the polyphenol-rich dietary pattern, we found a significant increase of microbial-diversity suggesting an improvement in the gut microbial ecosystem, a reduction of specific pro-inflammatory bacteria and an associated increase of protective microbial derived metabolites.

Mechanisms were investigated using cultured intestinal cell and animal models. Using very old mice, it was shown that supplementation with a mixture of polyphenols that were similar to those incorporated into the older human participant diets caused a significant reduction in IP. No decreases in IP were observed in the younger mice, suggesting that the polyphenol-mediated effects are restricted to the very old animals. The polyphenol-supplemented diet did not change the gut microbiota structure or faecal metabolite profiles and the gut immune response to a flagellin challenge. Using cultured intestinal cells, it was shown that specific flavonoids caused rapid and sustained increases in barrier function. In addition, specific polyphenols caused significant changes in the secretion of inflammatory cytokines in response to flagellin, a bacteria-derived inflammatory challenge.

The MaPLE project will generate new data for the exploitation in terms of future dietary guidelines that can be easily applied in different contexts such as nursing homes or for the support in the management of different clinical conditions associated with increased IP.

Consortium

Partner Organization	Partner Country
Institute of Food Research (now Quadram Institute Bioscience - QIB)	United Kingdom
University of Barcelona (UB)	Spain

Highlights

Aging is a societal challenge that need to be faced through multi target strategies including the important contribution of an adequate diet to healthy aging. Experimental evidence suggests that an alteration in human intestinal barrier properties can occur during aging. In this regard, the Maple project has produced several important findings and possibility of exploitation in different contexts as it is reported below.

Intestinal permeability appeared to be increased in aged people and should be considered as a potential target for screening (e.g. of increased risk of inflammation, metabolic disorders and associated clinical conditions)
Serum zonulin, a protein involved in the regulation of intercellular junctions, was correlated with circulating microbial DNA suggesting these as potential future evaluation as candidate biomarkers for age-related clinical conditions
A polyphenol-rich diet had a positive impact on IP in a group of older subjects, and these findings were supported by similar findings in an animal model of the ageing gut
We have generated evidence to support a plausible (but as yet unproven) mechanism of action, i.e. that the beneficial effects of the PR diet on IP were the result of decreases in a group of pro-inflammatory and IP-associated bacteria, and these changes were also associated with changes in important bacterial metabolites (SCFAs) associated with a healthy gut.
In vitro, specific polyphenols were shown to (i) cause rapid and sustained increases in intestinal barrier function (flavonoid-induced increases in TEER) and (ii) reduce the expression of inflammatory cytokines such as IL-8 in flagellin-challenged CaCo2 cells (quercetin and genistein, but not epigallocatechin gallate).
An increased polyphenol intake caused also a reduction in diastolic blood pressure and the effect was stronger in women.
We generated data that boost the study of polyphenol metabolites derived from bacterial activity, in order to reflect the microbiota's footprint on nutritional biomarkers.
The inhibition of the intestinal enzyme PARP1 by specific methylxanthine metabolites and microbial derived polyphenol metabolites could explain, at least in part, and at molecular scale, the positive effects of the MaPLE diet on the intestinal permeability.
The development of an in-house polyphenol database will support estimations of the intakes of different classes of and individual polyphenols, and their food sources, in future cohort and RCT studies. It will also underpin efforts to develop new targeted approaches for dietary recommendations in older adults.
Our study demonstrated that older subjects are available to include food products in their diet if this does not change dramatically their dietary habits. The results will impact on future dietary guidelines, the development of new food products and translational research including efforts to increase awareness of the general public.

Products

Title: Systematic review on polyphenol intake and health outcomes: is there sufficient evidence to define a health-promoting polyphenol-rich dietary pattern?
Author: Del Bo' C (UMIL), Bernardi S (UMIL), Marino M (UMIL), Porrini M (UMIL), Tucci M (UMIL), Guglielmetti S (UMIL), Cherubini A (INRCA linked to UMIL), Carrieri B (INRCA linked to UMIL), Kirkup B (QIB), Kroon PA (QIB), Zamora-Ros R (UB), Hidalgo Liberona N (UB), Andres-Lacueva C (UB), Riso P (UMIL)
Link: https://doi.org/10.3390/nu11061355

Title: Polyphenols and Intestinal Permeability: Rationale and Future Perspectives.
Author: Bernardi S (UMIL), Del Bo' C (UMIL), Marino M (UMIL), Gargari G (UMIL), Cherubini A (INRCA linked to UMIL), Andrés-Lacueva C (UB), Hidalgo-Liberona N (UB), Peron G (UB), González-Dominguez R (UB), Kroon P (QIB), Kirkup B (QIB), Porrini M (UMIL), Guglielmetti S (UMIL), Riso P (UMIL).
Link: https://doi.org/10.1021/acs.jafc.9b02283

Title: Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications.
Author: Peron G (UB), Hidalgo-Liberona N (UB), González-Domínguez R (UB), Garcia-Aloy M (UB), Guglielmetti S (UMIL), Bernardi S (UMIL), Kirkup B (QIB), Kroon PA (QIB), Cherubini A (INRCA linked to UMIL), Riso P (UMIL), Andrés-Lacueva C (UB).
Link: https://doi.org/10.1021/acs.jafc.9b01687

Title: Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial.
Author: Guglielmetti S (UMIL), Bernardi S (UMIL), Del Bo' C (UMIL), Cherubini A (INRCA linked to UMIL), Porrini M (UMIL), Gargari G (UMIL), Hidalgo-Liberona N (UB), Gonzalez-Dominguez R (UB), Peron G (UB), Zamora-Ros R (UB), Winterbone MS (QIB), Kirkup B (QIB), Kroon PA (QIB), Andres-Lacueva C (UB), Riso P(UMIL)
Link: https://doi.org/10.1186/s12877-020-1472-9

Title: Quantitative Dietary Fingerprinting (QDF)-A Novel Tool for Comprehensive Dietary Assessment Based on Urinary Nutrimetabolomics.
Author: Gonzalez-Dominguez R (UB), Urpi-Sarda M (UB), Jauregui O (UB), Needs PW (QIB), Kroon PA (QIB), Andres-Lacueva C (UB)
Link: https://doi.org/10.1021/acs.jafc.8b07023

Title: Bacterial DNAemia is associated with serum zonulin levels in older subjects.
Author: Gargari G (UMIL), Taverniti V (UMIL), Del Bo’C (UMIL), Bernardi S (UMIL), Andres-Lacueva C (UB), González-Domínguez R (UB), Kroon PA (QIB), Winterbone MS (QIB), Cherubini A (INRCA linked to UMIL), Riso P (UMIL), Guglielmetti S (UMIL).
Link: https://doi.org/10.1038/s41598-021-90476-0

Title: Role of a Polyphenol-Rich Dietary Pattern in the Modulation of Intestinal Permeability in Older Subjects: The MaPLE Study.
Author: Bernardi S (UMIL), Del Bo’ C (UMIL), Guglielmetti S (UMIL), A Cherubini (INRCA linked to UMIL), Kroon PA (QIB), Kirkup B (QIB), Hidalgo Liberona N (UB), Peron G (UB), González-Domíinguez R (UB), Andrés-Lacueva C (UB), Riso P (UB).
Link: https://doi.org/10.3390/proceedings2019011008

Title: FOBI: An ontology to represent food intake data and associate it with metabolomic data.
Author: Castellano-Escuder, P (UB); González-Domínguez, R (UB); Wishart, DS (UB); Andrés-Lacueva, C (UB), Sánchez-Pla, A (UB).
Link: https://doi.org/10.1093/databa/baaa033

Title: Role of berries in vascular function: a systematic review of human intervention studies.
Author: Martini D (UMIL), Marino M (UMIL), Angelino D, Del Bo' C (UMIL), Del Rio D, Riso P (UMIL), Porrini M (UMIL).
Link: https://doi.org/10.1093/nutrit/nuz053

Title: Modulation of Adhesion Process, E-Selectin and VEGF Production by Anthocyanins and Their Metabolites in an in vitro Model of Atherosclerosis.
Author: Marino M (UMIL), Del Bo' C (UMIL), Tucci M (UMIL), Klimis-Zacas D, Riso P (UMIL), Porrini M (UMIL).
Link: https://doi.org/10.3390/nu12030655

Title: A simple and rapid LC-MS/MS method for quantification of total daidzein, genistein and equol in urine.
Author: Saha S (QIB), Kroon PA (QIB).
Link: https://doi.org/10.1155/2020/2359397

Title: Metabolic signature of a functional high-catechin tea after acute and sustained consumption in healthy volunteers through 1H NMR-based metabolomics analysis of urine.
Author: Madrid-Gambin F (UB), Garcia-Aloy M (UB), Vázquez-Fresno R (UB), Vegas-Lozano E (UB), Sánchez-Pla A, Misawa K, Hase T, Shimotoyodome A, Andres-Lacueva C (UB).
Link: https://doi.org/10.1021/acs.jafc.8b04198

Title: Urinary TMAO Levels Are Associated with the Taxonomic Composition of the Gut Microbiota and with the Choline TMA-Lyase Gene (cutC) Harbored by Enterobacteriaceae.
Author: Dalla Via A (UMIL), Gargari G (UMIL), Taverniti V (UMIL), Rondini G, Velardi I, Gambaro V, Visconti GL, De Vitis V, Gardana C (UMIL), Ragg E, Pinto A, Riso P (UMIL), Guglielmetti S (UMIL). , Ragg E, Pinto A, Riso P (UMIL), Guglielmetti S (UMIL).
Link: https://doi.org/10.3390/nu12010062

Title: Exploring Associations between Interindividual Differences in Taste Perception, Oral Microbiota Composition, and Reported Food Intake.
Author: Cattaneo C, Riso P (UMIL), Laureati M, Gargari G (UMIL), Pagliarini E.
Link: https://doi.org/10.3390/nu11051167

Title: Overview of Human Intervention Studies Evaluating the Impact of the Mediterranean Diet on Markers of DNA Damage.
Author: Del Bo' C (UMIL), Marino M (UMIL), Martini D (UMIL), Tucci M (UMIL), Ciappellano S (UMIL), Riso P (UMIL), Porrini M (UMIL).
Link: https://doi.org/10.3390/nu11020391

Title: Anthocyanins and metabolites resolve TNF-α-mediated production of E-selectin and adhesion of monocytes to endothelial cells.
Author: Del Bo' C (UMIL), Marino M (UMIL), Riso P (UMIL), Møller P, Porrini M (UMIL).
Link: https://doi.org/10.1016/j.cbi.2019.01.002

Title: Lipids and physical function in older adults.
Author: Casas-Agustench P (UB), Cherubini A (INRCA linked to UMIL), Andrés-Lacueva C (UB).
Link: https://doi.org/10.1097/mco.0000000000000333

Title: Biomarkers of food intake for nuts and vegetable oils: an extensive literature search.
Author: Garcia-Aloy (UB) M, Hulshof PJM, Estruel-Amades S, Osté, MCJ, Lankinen M, Geleijnse JM, de Goede J, Ulaszewska M, Mattivi F, Bakker SJL, Schwab U, Andres-Lacueva C (UB).
Link: https://doi.org/10.1186/s12263-019-0628-8

Reports

Endreport

Summary

All the results obtained from the MaPLE project will be fundamental to support the importance of healthy dietary patterns rich in polyphenols on the modulation of microbiome and its impact on leaky gut in older subjects.

Therefore, researchers will have preliminary data to plan large scale studies on polyphenol and healthy aging. Moreover, clinicians (geriatricians, nurses, nutritionists, etc) will be able to use the results to better tailor interventions for prevention and treatment of illnesses related to intestinal permeability. Consequently, results obtained can be considered important to provide further evidence for improving the older subject’s quality of life and promoting a protective metabolic phenotype in this target group.

In addition, we strongly believe that results obtained in the older population can be pivotal for the application and exploitation in other sensible groups of the population where impairment of the intestinal barrier function may be an important target.

This project has provided a number of results that directly contribute to the understanding of the relationships between markers of gut function (intestinal permeability, intestinal inflammation), gut microbiota structure (16S rRNA gene profiling), gut function (intestinal content metabolite profiles) and host health status (peripheral inflammation, blood bacterial load, circulating metabolite profiles). In addition, the project has revealed how these markers respond to a polyphenol-enriched diet.

We have also shown that the quantification and taxonomic profiling of bacterial DNA circulating in blood (blood microbiomics) could be of strategic importance because they may reflect the link between intestinal barrier functionality, and the local and systemic immunity. Considering that most chronic-degenerative diseases are based on immune system dysregulation and intestinal mucosal homeostasis impairment, our results promote future research able to validate blood microbiomics as a promising molecular tool for screening pre-pathological physiological states.

Results

One strategic goal of the JPI is the support and promotion of research and strategies to change dietary patterns and to develop evidence-based recommendations and innovative products that can have a major impact on improving public health, increasing the quality of life in all the life stages. In this context, Maple project has given a significant contribution since we have shown that consuming a polyphenol-rich dietary pattern it is possible to improve intestinal permeability (IP) in the older subjects possibly due to beneficial changes in the structure and function of the gut microbiome. Our results can promote further research and development to develop non-pharmacological strategies to improve IP and related disorders not only in the older population but also in other vulnerable target groups suffering from increased IP and/or associated inflammatory conditions. Within our project we have also tried to provide exploitation of our results to highlight potential existing gaps and promote the development of future dietary guidelines for the older subjects (e.g. in the residential care settings). Moreover, our results have provided an initial proof of concept that can be also useful in underpinning R&D activities and exploitation routes for other aspects related to food implementation, dietary protocols development, new tools and markers for IP screening. In this regard, we have proposed for the first time, bacterial DNAemia as a candidate biomarker helpful for the prognosis and/or prediction of metabolic and clinical pathologic conditions in the older subjects by considering the correlations found with zonulin levels and inflammatory markers. Finally, by considering the relevance of the implementation of data sharing, as another strategic JPI HDHL main goal, MaPLE consortium have become members of the the new HDHL-INTIMIC Knowledge Platform on Food, Diet, Intestinal Microbiomics and Human Health in which will contribute with knowledge developed through the projects and further exploitation of data obtained to disentangle the role of gut microbiome on the response to polyphenol rich diets.

Communication & Dissemination Activities

Target group	Authors	Means of communication
Scientists (mainly), food companies	P. Riso (UMIL), S. Guglielmetti (UMIL), M. Porrini (UMIL), D. Mora (UMIL), A. Cherubini (INRCA-linked to UMIL), C. Andres-Lacueva (UB), M. Rabassa (UB), M. Urpi-Sarda (UB), P. Casas Agustench (UB), A. Narbad (QIB), M. S. Winterbone (QIB), C. Nicoletti, P. Kroon (QIB), “Gut and blood microbiomics for studying the effect of a polyphenol-rich dietary pattern on intestinal permeability in the elderly”, Food Bioactives & Health Conference FBHC, 13-15 September 2016, Norwich, United Kingdom,	Poster presentation
Host and staff of the nursing homes-OIC Foundation	Riso P (UMIL): “Progetto polifenoli: mangiare bene per vivere meglio”, Civitas Vitae - OIC Foundation (nursing homes and residencies for older subjects), Padua Italy, 2016	Project presentation through keynote and talk/discussion
Physicians and care- givers	Riso P (UMIL): Gut and blood microbiomics for studying the effect of a polyphenol-rich dietary pattern on intestinal permeability in the elderly, OIC Foundation, Padua, Italy, 2016	Formal presentations of MaPLE objectives through keynote and talk/discussion
Older persons and their relatives	Riso P (UMIL): “Alimentazione e longevità” - Gut and blood microbiomics for studying the effect of a polyphenol-rich dietary pattern on intestinal permeability in the elderly, OIC Foundation, Padua, Italy, 2016	Presentations and seminars on MaPLE project approach
Scientists and nutrition professionists/clinicians (mainly), food and nutraceuticals companies	P. Riso (UMIL), Simone Guglielmetti (UMIL), Marisa Porrini (UMIL), Cristian Del Bo’ (UMIL), Diego Mora (UMIL), Antonio Cherubini (INRCA-linked to UMIL), Cristina Andres-Lacueva (UB), Nicole Hidalgo (UB), Patricia Casas-Agustench (UB), Montserrat Rabassa (UB), Arjan Narbad (QIB), Claudio Nicoletti, Mark S Winterbone (QIB),Paul A. Kroon (QIB), MaPLE project: gut and blood microbiomics for studying the effect of a polyphenol-rich dietary pattern on intestinal permeability in the elderly, National Congress of the Italian Society of Human Nutrition, SINU 30 November-2 December 2016, Bologna, Italy	Poster presentation
Participants to the project including volunteers and their families, care-givers, clinicians and all staff at the nursing homes	Riso P and UMIL group, OIC Foundation staff – Award Celebration, 2017	General talk and celebration of end of intervention with attribution of awards to participants
Scientists (mainly), food and nutraceutical companies	S. Bernardi (UMIL), S. Guglielmetti (UMIL), C. Del Bo’ (UMIL), M. Marino (UMIL); G. Gargari (UMIL), A. Cherubini (INRCA-linked to UMIL), C. Andres-Lacueva (UB), N. Hidalgo (UB), P. Casas- Agustench (UB) , M. Winterbone (QIB), A. Narbad (QIB), P. Kroon (QIB), P. Riso (UMIL). A randomised controlled trial to assess the impact of a polyphenol-enriched diet on intestinal permeability in the elderly: The MaPLE study, ICPH 3-6 October 2017, Quebec City, Canada	poster presentation
Scientists (mainly), food and nutraceutical companies	Cristina Andres-Lacueva (UB), Frailty, Polyphenols and Healthy Aging”, ICPH 2017, Quebec city	Keynote (oral presentation)
Scientists	Cristina Andres-Lacueva (UB). Polyphenols and gut microbiome for a healthy aging. Evidence from clinical studies. IUNS International Congress of Nutrition, Buenos Aires, October 2017.	Oral communication
Scientists and nutrition professionists/clinicians (mainly), food and nutraceuticals companies	S. Bernardi (UMIL), S. Guglielmetti (UMIL), C. Del Bo’ (UMIL), G. Gargari (UMIL), A. Cherubini (INRCA-linked to UMIL), C. Andres-Lacueva (UB), P. Kroon (QIB), P. Riso (UMIL). Valutazione della permeabilità intestinale in un gruppo di soggetti anziani nell’ambito del progetto MaPLE, National Congress of the Italian Society of Human Nutrition, SINU 20-22 November 2017, Torino, Italy	poster presentation
Scientists (mainly), food, nutraceutical and analytical companies	C. Del Bo’ (UMIL), S. Bernardi (UMIL), S. Guglielmetti (UMIL), G. Gargari (UMIL), A. Cherubini ((INRCA-linked to UMIL), C. Andres-Lacueva (UB), P. Kroon (QIB), P. Riso (UMIL). Evaluation of intestinal permeability in a group of older subjects participating to the MaPLE randomised controlled trial, Foodomics 10-12 January 2018, Cesena, Italy	poster presentation
Geriatricians and Scientists	A. Cherubini (INRCA-linked to UMIL), S. Guglielmetti (UMIL), A.P. Kroon (QIB), C. Andres-Lacueva (UB), P. Riso (UMIL). The Diet-Microbiota-Intestinal Permeability axis in older subjects: rationale of the MaPLE Project 14th Congress 2018 of European Union Geriatric Medicine Society, 10-12 October, 2018 Berlin, Germany	Poster presentation
Scientists (mainly), food and nutraceutical companies	P. Riso (UMIL). A polyphenol-rich dietary pattern as a strategy to improve intestinal permeability: rationale and outcomes of the MaPLE project; FBHC 2018 Lisbon, 26-28 September 2018 Portugal	Oral presentation
Scientists (mainly, food and nutraceutical companies	C. Andres-Lacueva (UB). Nutritional Metabolomics: Dietary Evaluation from Biomarkers¸ FBHC 2018 Lisbon, 26-28 September 2018 Portugal	Oral presentation
Scientists (mainly), food and nutraceutical companies	S. Bernardi (UMIL), N. Hidalgo Liberona (UB), C. Del Bo’ (UMIL), S. Guglielmetti (UMIL), G. Gargari (UMIL), A. Cherubini (INRCA-linked to UMIL), P. Kroon (QIB), C. Andres-Lacueva (UB), P. Riso (UMIL). Estimation of the intake of bioactive compounds in older subjects in a nursing home setting - the MaPLE project, Food Bioactives and Human Health Conference FBHC 26-28 September 2018, Lisbon, Portugal	Poster presentation
Scientists and nutrition professionists/clinicians (mainly), food and nutraceuticals companies	S. Bernardi (UMIL), S. Guglielmetti (UMIL), G. Gargari (UMIL), A. Cherubini (INRCA-linked to UMIL), C. Andres-Lacueva (UB), N.Hidalgo Liberona (UB), P. Casas-Agustench (UB), P. Kroon (QIB), P. Riso (UMIL). Valutazione dell’assunzione di polifenoli nei soggetti anziani del progetto MaPLE, National Congress of the Italian Society of Human Nutrition, SINU 19-21 November 2018, Napoli, Italia	Poster presentation
Scientists	P. Riso (UMIL), S. Guglielmetti (UMIL), S. Bernardi (UMIL), G. Gargari (UMIL), A. Cherubini (INRCA-linked to UMIL), P. Kroon (QIB), C. Andres-Lacueva (UB). Rationale of MaPLE project focused on intestinal permeability in the older subjects, COST action CA 16112 “Personalized Nutrition in aging society: redox control of major age-related diseases” Workshop, 19-21 September 2018, Gdansk, Poland	Oral and poster presentation
Scientists (mainly), food companies	P. Riso (UMIL). Role of food bioactives in the modulation of intestinal permeability: insights from the MaPLE study and beyond. COST action CA15136 “European network to advance carotenoid research and applications in agro-food and health – Eurocaroten” Workshop, 23-24 October 2018, Valencia, Spain	Oral presentation
Scientists, companies	G. Gargari (UMIL), V. Taverniti (UMIL), C. Del Bo’ (UMIL), S. Bernardi (UMIL), C. Andres-Lacueva (UB), P.A. Kroon (QIB), M.S. Winterbone (QIB), A. Cherubini (INRCA-linked to UMIL), P. Riso (UMIL), S. Guglielmetti (UMIL). Bacterial DNA in blood is associated with serum zonulin levels in older subjects, Targeting Microbiota 28th-30th October 2018, Porto, Portugal	Poster presentation
Geriatricians and clinicians	A. Cherubini (INRCA-linked to UMIL), S. Guglielmetti (UMIL), A.P. Kroon (QIB), C. Andres-Lacueva (UB), P. Riso (UMIL). Ruolo della dieta nella modulazione della permeabilità intestinale nell’anziano: il progetto MaPLE. Annual Congress of the Italian Society of Gerontology and Geriatrics (SIGG), Rome 28 November – 1 December 2018	Oral presentation
Scientists	S. Bernardi (UMIL), C. Del Bo’ (UMIL), S. Guglielmetti (UMIL), A. Cherubini (INRCA-linked to UMIL), A.P Kroon (QIB), B. Kirkup (QIB), N. Hidalgo Liberona (UB), G. Peron (UB), R. González-Domíinguez (UB), C. Andrés-Lacueva (UB), P. Riso (UMIL). Role of a Polyphenol-Rich Dietary Pattern in the Modulation of Intestinal Permeability in Older Subjects: The MaPLE Study. Natural Products and the Hallmarks of Chronic Diseases - COST Action 16112- Personalized Nutrition in aging society: redox control of major age-related diseases, 25-27 March 2019, Luxemburg.	Poster presentation
Geriatricians and clinicians	A. Cherubini (INRCA-linked to UMIL), S. Bernardi (UMIL), C. Del Bo’ (UMIL), S. Guglielmetti (UMIL), G. Gargari (UMIL), A.P. Kroon (QIB), B. Kirkup (QIB), B. Carrieri (INRCA-linked to UMIL), N. Hidalgo Liberona (UB), G. Peron (UB), R. Gonzalez Dominguez (UB), C. Andres-Lacueva (UB), P. Riso (UB). Role of a polyphenol-rich dietary pattern in the modulation of intestinal permeability and related markers in the older subjects. International Conference of the European Geriatrics Medicine Society EUGMS 25-27 September 2019, Krakov, Poland	Oral presentation
Scientists	Castellano-Escuder P (UB), González-Domínguez R (UB), Wishart DS, Andrés-Lacueva C (UB), Sánchez-Pla A. FOBI: An ontology to represent food intake data and associate it with metabolomic data. Nordic Metabolomics Society Workshop and Thone Holst Symposium. October 31st - November 1st, 2019 (Oslo, Norway)	Poster persentation
Scientists, Nutritionists, Dieticians, Food companies, Associations	S. Bernardi (UMIL), C. Del Bo’ (UMIL), S. Guglielmetti (UMIL), A. Cherubini (INRCA-linked to UMIL), A.P. Kroon (QIB), B. Kirkup (QIB), N. Hidalgo Liberona (UB), G. Peron (UB), R. Gonzalez Dominguez (UB), C. Andres-Lacueva (UB), P. Riso (UMIL), Intestinal permeability modulation through a polyphenol-rich dietary pattern in the older subjects. MaPLE project outcomes and perspectives, 13th European Nutrition Conference FENS, 15-18 October 2019, Dublin, Ireland	e-poster presentation
Scientists (mainly), food and nutraceutical companies	P. Riso (UMIL), S. Bernardi (UMIL), C. Del Bo’ (UMIL), P. Kroon (QIB), B. Kirkup (QIB), A. Cherubini (INRCA-linked to UMIL), N. Hidalgo Liberona (UB), G. Peron (UB), R. González-Dominguez (UB), C. Andrés-Lacueva (UB), S. Guglielmetti (UMIL). MaPLE study: reliability and significance of a polyphenol-rich dietary pattern for the improvement of intestinal permeability in the older subjects, The 9th International Conference on Polyphenols and Health ICPH, 28th November-1st December, 2019 Kobe, Japan	Poster presentation
Scientists (mainly), food and nutriaceutical companies	C Andres-Lacueva (UB). Metabolomics based dietary biomarkers in Nutritional Epidemiology. Challenges and opportunities in polyphenol research. The 9th International Conference on Polyphenols and Health ICPH, 28th November-1st December, 2019 Kobe, Japan	Oral communications
Scientists, nutrition experts/clinicians (mainly), food and nutraceutical companies	S. Bernardi (UMIL), C. Del Bo’ (UMIL), G. Gargari (UMIL), A. Cherubini (INRCA-linked to UMIL), A.P. Kroon (QIB), N. Hidalgo-Liberona (UB), C. Andrès-Lacueva (UB), S. Guglielmetti (UMIL), M. Porrini (UMIL), P. Riso (UMIL). Effetto di una dieta ricca in polifenoli sulla permeabilità intestinale in un gruppo di soggetti anziani: il progetto MaPLE. National Congress of the Italian Society of Human Nutrition (SINU), 27-29 November 2019, Genova, Italy	Oral communication
Geriatrician and clinicians	Cherubini A (INRCA-linked to UMIL). Bernardi, S (UMIL)., Del Bo’, C (UMIL). , Guglielmetti, S (UMIL)., Gargari, G (UMIL)., Kroon, P (QIB)., Kirkup, B (QIB)., Carrieri, B.(INRCA-linked to UMIL) ,Hidalgo Liberona, N. (UB), Peron, G. (UB), Gonzalez Dominguez, R. (UB), Andres-Lacueva, C. (UB), Riso, P (UMIL). Effetti di una dieta ricca di polifenoli nella modulazione della permeabilità intestinale e dei livelli di marcatori correlati in soggetti anziani. In 64th Annual Congress of the Italian Society of Gerontology and Geriatrics (SIGG), Rome 28 November 2019	Oral communication
Scientists	C. Del Bo’ (UMIL), S. Bernardi (UMIL), M. Marino (UMIL), L. Gigliotti (UMIL), A. Cherubini (INRCA-linked to UMIL), M. Porrini (UMIL), N. Hidalgo-Liberona (UB), R. Gonzalez-Dominguez (UB), R. Zamora-Ros (UB), G. Peron (UB), B. Kirkup (QIB), P.A. Kroon (QIB), C. Andres-Lacueva (UB), S. Guglielmetti (UMIL), P. Riso (UMIL). Effect of a polyphenol-rich diet on markers of intestinal permeability and oxidative stress in older subjects: results from the MaPLE project, Human Biomonitoring and the Comet Assay 27 March 2020, Budapest, Hungary	Poster presentation (postponed Croatia, 2-3 October 2020)
Scientists (mainly), food and nutraceutical companies	C. Del Bo’ (UMIL), A. Cherubini (INRCA-linked to UMIL), P.A. Kroon (QIB), C. Andres-Lacueva (UB), S. Guglielmetti (UMIL), P. Riso (UMIL). Modulation of intestinal permeability through dietary polyphenols: teaching from the MaPLE study, FBHC 30 November-4 December 2020, Parma, Italy	Oral presentation (postponed 30 November - 4 December 2020)
Scientists (mainly), food and nutraceutical companies	S. Bernardi (UMIL), M. Marino (UMIL), L. Gigliotti (UMIL), C. Del Bo’ (UMIL), A. Cherubini (INRCA-linked to UMIL), M. Porrini (UMIL), N. Hidalgo-Liberona (UB), R. Gonzalez-Dominguez (UB), R. Zamora-Ros (UB), G. Peron (UB), B. Kirkup (QIB), P.A. Kroon (QIB), C. Andres-Lacueva (UB), S. Guglielmetti (UMIL), P. Riso (UMIL), Role of a polyphenol-rich foods in the modulation of DNA damage as oxidative stress marker in older subjects, FBHC 30 November-4 December 2020, Parma, Italy	Poster presentation (postponed 30 November - 4 December 2020)
Scientists (mainly), food and nutraceutical companies	G. Gargari (UMIL), V. Taverniti (UMIL), C. Del Bo’ (UMIL), S. Bernardi (UMIL), C. Andres-Lacueva (UB), R. González-Domíngue (UB), P.A. Kroon (QIB), M.S. Winterbone (QIB), A. Cherubini (INRCA-linked to UMIL), P. Riso (UMIL), S. Guglielmetti (UMIL). Bacterial DNAemia is associated with serum zonulin levels in older subjects involved in a dietary intervention based on high-polyphenol intake, 30 November-4 December 2020, Parma, Italy	Oral communication (postponed 30 November - 4 December 2020)
PhD students of the PhD Course in Food Systems (University of Milan)	Guglielmetti S (UMIL) and Riso P (UMIL), “The intestinal microbiota: interactions with host and diet”, Presentation of MaPLE rationale and approach within the general topic for future perspective. University of Milan, June 2016 and 2017	Oral presentation (Lectures)
PhD students of the PhD Course in Food Systems (University of Milan)	Cristian Del Bo’ (UMIL) and Riso P (UMIL). “Food Bioactives” Presentation of impact of food bioactives exploitation in different target group for the modulation of markers associated to human health including intestinal permeability. University of Milan, July 2019	Oral presentation (Lectures)

Features

Project number:: MaPLE
Duration: 100%: 2016

2019
Related funding round:: Intestinal Microbiomics (2015)
Project lead and secretary:: Patrizia Riso

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